I do have some preserved Gram Stains of the bacteria before and after the dosing cycle. Those are cheap do as I have a freind who runs a pediatricians office and he gave me the stuff to do them. I do know there is a big increase in Bacteria a month into the cycle. You get the same spike in BioOptim and BioDigest. Like I said I don't know the identity of each item on the Gram stain. I would like to send them away to get stuff identified but its pretty expensive. I for one would like to see some convincing science from ZEO, Prodibio, and Polyp-Labs. That their system selectively only increases the positive Bacteria. I know that these systems have you dose the bacs that they say are good and then have you dose a carbon fuel for them to multiple. Still I dont think there is anyway to know what exactly your doing with carbon dosing of Any kind. I have not had problems as of yet with either Prodibio or sugar. I question however the feeding aspect of these system. I am not sure if either is worth the risk to provide a food source for coral. I will still continue to use Sugar because I know that it allows me to over feed. I've tried it both ways. Without the sugar I get Nitrate spikes. Currently with the pappone feeding method I think that the sugar is the best way to go. Thats why its in the pappone food.