I have had my tank up and running for about 6 months now. My nitrates are reading 25ppm and i haven't changed my set up at all. I have my heater in chamber one. In chamber two I have inTank media basket with floss, purigen, and chempure elite. I change my floss every other day. I also took out the sponge in chamber 3. I do a water change once a week If anyone has suggestions that would be great.