Dr. Tim, I am getting ready to get your One and Only bacteria. I am setting up 180 gallon with sump and macro algae DT (refugium). The total volume is close to 250g. I have seen Mr. Saltwater video about your product. He mentioned that anything above 75g, I really dont need to supply ammonia. Instead, fish will help producing ammonia which will feed the bacteria. My questions are: 1. How many fish should I get to help some ammonia in 250g? 2. Can I start corals right away? 3. What kind of equipment I should turn on/off? 4. How long is the shelf life of this product? Thank you so much.