How long to your acclimate your fish? What method of acclimation do you follow? I float bag in tank for 10 - 15 minutes. Then start adding a couple handfuls aquarium water to the bag every 10-15 minutes up to 45 minutes. Then I dump out the water through a net to catch the fish and add it to the tank. Doing the net thing can stress the fish out a bit, but I'd rather do that than add the LFS water to my tank. Sometimes their tanks are copper-treated and that's not something you want to add to your reef.