I usually temp acclimate the bag for at least an hour before I start water acclimating the fish. I generally will add 2-3cc's of tank water into the bag every 30 mins with my pipettes. Hardy fish usually take me about 2-2.5 hours to acclimate, and more sensitive animals will take the better half of 4 hours. I took almost 6 hours to introduce my anemone into the tank. We can be in a hurry to add them into the tank, but we have to fight that urge and take it slow. Patience is key, especially since their lives are in our hands. Even when our tanks are pristine, it doesn't mean that the tanks they lived in at the LFS are equally as nice. Going from an "ok" tank to a clean tank can be just as big of a shock as going from a clean tank to an "ok" tank.